GDF-5 promotes epidermal stem cells proliferation via Foxg1-cyclin D1 signaling

Abstract Objective Epidermal stem cells (EpSCs) can self-renew, which are responsible for the long-term maintenance of skin, and it also plays a critical role in wound re-epithelization, but mechanism underlying EpSCs proliferation is unclear. GDF-5, known as BMP-14, member BMP family be used self-renewal supporter. Here, we studied effects GDF-5 on mouse healing. Methods Firstly, was tested by using CCK8 reagent PCNA expression analyzed Western blotting. Secondly, screened genes that promote FOX cyclin qPCR, then protein level selected further Thirdly, siRNA plasmids pAdEasy adenovirus were transfected or infected, respectively, into to detect effect target GDF-5-induced cell proliferation. Furthermore, injected deep partial thickness burn model finding out whether detected immunohistochemical. Finally, relevant immunoblotting, dual-luciferase reporter gene detection. Results We discovered 100 ng/ml recombinant optimal concentration promoting Through preliminary found Foxg1 D1 could downstream molecules results confirmed And adjusted Foxg1/cyclin vitro vivo. Besides, transcription regulated Foxg1-mediated promoter activity. Conclusion This paper showed promotes via Foxg1-cyclin signal pathway. It suggested may new approach make